Influence of Sacarose and Sodium Chloride in the Toxicity Evaluation of Environmental Samples Using V. Fischeri

Authors

  • G. A. Umbuzeiro Faculdade de Tecnologia, universidade estadual de Campinas – uNICAmP,
  • E. M. Hachich Companhia Ambiental do estado de São Paulo – CeTeSB
  • E. V. F. Magri Companhia Ambiental do estado de São Paulo – CeTeSB
  • S. H. K. Takeda Companhia Ambiental do estado de São Paulo – CeTeSB
  • M. I. Z. Sato Companhia Ambiental do estado de São Paulo – CeTeSB

Abstract

The acute toxicity assay using the marine bacteria Vibrio fischeri requires osmotic adjustment for testing samples with low salt content. Two kinds of compounds can be used for this purpose, sodium chloride (NaCl) or sucrose. It is known that this osmotic adjustment can cause changes in the toxicity for some substances like some metals and ammonia. The toxicity of 29 environmental samples that included raw waters, industrial effluents, sediments, solid wastes and foams deriving from polluted water bodies was evaluated using NaCl and sucrose, in parallel. The two methods of osmotic adjustment showed a good agreement in terms of the qualitative responses for all samples analyzed. Only for five samples, the CE50 values were lower in sucrose than in NaCl, in agreement with the results of zinc and ammonia. The results obtained for the environmental samples tested with both methods suggest that NaCl can be used routinely. Additional analysis with sucrose can be especially helpful when metals like zinc and ammonia are suspected to be the key toxicant in the samples.

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How to Cite

Umbuzeiro, G. A., Hachich, E. M., Magri, E. V. F., Takeda, S. H. K., & Sato, M. I. Z. (2011). Influence of Sacarose and Sodium Chloride in the Toxicity Evaluation of Environmental Samples Using V. Fischeri. Ecotoxicology and Environmental Contamination, 5(1). Retrieved from https://periodicos.univali.br/index.php/eec/article/view/3047

Issue

Vol. 5 No. 1 (2010)

Section

Original Articles

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Copyright © 2006 ECOTOX-Brasil

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