Review of embryo-larval assay of the mussel Perna perna (L.)

Claudiane Carla Del Cielo, Jessica Malgarin, Charrid Resgalla

Abstract


The objective of this work was to review the protocol of the mussel Perna perna embryo-larval test and suggest standardization of the induction technique, exposing time and effect observed in larvae at the end of the experiment. To meet the objectives were employed different physical and chemical methods to accelerate the release of gametes and the success of this release was compared to the index condition of the organisms. Ecotoxicological tests were also performed using the metals cadmium, lead, zinc, copper, chromium and the detergent sodium dodecyl sulfite (SDS) to assess the exposure time and morphological types of effects and their influence on estimates of Median Effective Concentration (EC50). The most efficient induction method to stimulate the release of gametes was the continuous flow system of sea water, but it had a close relationship with the maturation of organisms. It was observed that the short term embryo-larval test can be executed in 24 hours since protracted larvae are considered normal as observed in control flasks experiments. Finally, embryo-larval test of the P. perna showed sensitivity in the same order of magnitude as compared to Lytechinus variegatus the main species of sea urchin commonly used in marine chronic tests in Brazil.


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DOI: http://dx.doi.org/10.5132/eec.2013.02.006

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